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anti fibroblast microbeads  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec anti fibroblast microbeads
    Anti Fibroblast Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+anti+fibroblast+microbeads/Anti-Fibroblast+MicroBeads%2C+human/us12668765-85-15-17
    Average 95 stars, based on 83 article reviews
    anti fibroblast microbeads - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Suspension:

    Article Title: SGLT2 inhibitors attenuate endothelial to mesenchymal transition and cardiac fibroblast activation
    Article Snippet: Trypsinized cells were washed and resuspended in MACS buffer, i.e. BSA and autoMACSTM rinsing solution (Miltenyi Biotec) both 1:20 in ddH 2 O. .. One quarter of the of the cell suspension volume human anti-fibroblast Microbeads (Miltenyi Biotec) were added for 30 min at RT, light protected. .. Afterwards, two washing steps with 1 mL MACS buffer were performed before filtering the suspension through a 30 μm filter onto an MS column located in a suitable MACS separator (all Miltenyi Biotec), which had been rinsed with 500 μL MACS buffer before.

    Article Title: Endosome associated trafficking regulator 1 promotes tumor growth and invasion of glioblastoma multiforme via inhibiting TNF signaling pathway.
    Article Snippet: Briefly, tumor specimens were washed with phosphate buffered saline (PBS, Gibco, United States, #70011069) and then enzymatically digested by using human Tumor Dissociation kit (Miltenyi Biotec, Germany, #130-095-929). .. Fibroblasts containing in the cell suspension were removed by using human Anti-Fibroblast MicroBeads (Miltenyi Biotec, Germany, #130-050-601) and erythrocytes were removed by using Red Blood Cell Lysis Solution (10×) (Miltenyi Biotec, Germany, #130-094-183). ..

    Article Title: Targeting OxLDL-mediated CD36 + CAF reprogramming potentiates PD-1 immunotherapy in osteosarcoma.
    Article Snippet: .. For human fibroblast isolation, 600 the Human Anti-Fibroblast MicroBeads (Miltenyi Biotec, #130-050-601) were incubated with the 601 AR TIC LE IN PR ES S single-cell suspension in PEB buffer at 4°C for 15 minutes. .. Positive selection was performed using 602 two sequential LS/MS separation columns (Miltenyi Biotec, Germany).

    Article Title: Targeting OxLDL-mediated CD36 + CAF reprogramming potentiates PD-1 immunotherapy in osteosarcoma
    Article Snippet: .. For human fibroblast isolation, the Human Anti-Fibroblast MicroBeads (Miltenyi Biotec, #130-050−601) were incubated with the single-cell suspension in PEB buffer at 4 °C for 15 min. .. Positive selection was performed using two sequential LS/MS separation columns (Miltenyi Biotec, Germany).

    Article Title: SGLT2 inhibitors attenuate endothelial to mesenchymal transition and cardiac fibroblast activation.
    Article Snippet: .. One quarter of the of the cell suspension volume human anti-fibroblast Microbeads (Miltenyi Biotec) were added for 30 min at RT, light protected. .. Afterwards, two washing steps with 1 mL MACS buffer were performed before filtering the suspension through a 30 μm filter onto an MS column located in a suitable MACS separator (all Miltenyi Biotec), which had been rinsed with 500 μL MACS buffer before.

    Article Title: Cancer-associated fibroblasts promote the malignant development of lung cancer through the FOXO1 protein/LIF signaling.
    Article Snippet: This paper aims to investigate the current situation of cancer related fibroblasts promoting malignant development of cancer through FOXO1 protein/LIF signal, and explore the strategy of cancer treatment.. Recent studies have shown that the expression of the protein forkhead box O1 (FOXO1) is increased in CAFsCAFs (Cancerassociated fibroblasts).. This led researchers to investigate whether FOXO1 is involved in the role of CAFs in lung cancer.

    Red Blood Cell Lysis:

    Article Title: Endosome associated trafficking regulator 1 promotes tumor growth and invasion of glioblastoma multiforme via inhibiting TNF signaling pathway.
    Article Snippet: Briefly, tumor specimens were washed with phosphate buffered saline (PBS, Gibco, United States, #70011069) and then enzymatically digested by using human Tumor Dissociation kit (Miltenyi Biotec, Germany, #130-095-929). .. Fibroblasts containing in the cell suspension were removed by using human Anti-Fibroblast MicroBeads (Miltenyi Biotec, Germany, #130-050-601) and erythrocytes were removed by using Red Blood Cell Lysis Solution (10×) (Miltenyi Biotec, Germany, #130-094-183). ..

    Isolation:

    Article Title: Targeting OxLDL-mediated CD36 + CAF reprogramming potentiates PD-1 immunotherapy in osteosarcoma.
    Article Snippet: .. For human fibroblast isolation, 600 the Human Anti-Fibroblast MicroBeads (Miltenyi Biotec, #130-050-601) were incubated with the 601 AR TIC LE IN PR ES S single-cell suspension in PEB buffer at 4°C for 15 minutes. .. Positive selection was performed using 602 two sequential LS/MS separation columns (Miltenyi Biotec, Germany).

    Article Title: Targeting OxLDL-mediated CD36 + CAF reprogramming potentiates PD-1 immunotherapy in osteosarcoma
    Article Snippet: .. For human fibroblast isolation, the Human Anti-Fibroblast MicroBeads (Miltenyi Biotec, #130-050−601) were incubated with the single-cell suspension in PEB buffer at 4 °C for 15 min. .. Positive selection was performed using two sequential LS/MS separation columns (Miltenyi Biotec, Germany).

    Article Title: Cancer-associated fibroblasts promote the malignant development of lung cancer through the FOXO1 protein/LIF signaling.
    Article Snippet: This paper aims to investigate the current situation of cancer related fibroblasts promoting malignant development of cancer through FOXO1 protein/LIF signal, and explore the strategy of cancer treatment.. Recent studies have shown that the expression of the protein forkhead box O1 (FOXO1) is increased in CAFsCAFs (Cancerassociated fibroblasts).. This led researchers to investigate whether FOXO1 is involved in the role of CAFs in lung cancer.

    Incubation:

    Article Title: Targeting OxLDL-mediated CD36 + CAF reprogramming potentiates PD-1 immunotherapy in osteosarcoma.
    Article Snippet: .. For human fibroblast isolation, 600 the Human Anti-Fibroblast MicroBeads (Miltenyi Biotec, #130-050-601) were incubated with the 601 AR TIC LE IN PR ES S single-cell suspension in PEB buffer at 4°C for 15 minutes. .. Positive selection was performed using 602 two sequential LS/MS separation columns (Miltenyi Biotec, Germany).

    Article Title: Targeting OxLDL-mediated CD36 + CAF reprogramming potentiates PD-1 immunotherapy in osteosarcoma
    Article Snippet: .. For human fibroblast isolation, the Human Anti-Fibroblast MicroBeads (Miltenyi Biotec, #130-050−601) were incubated with the single-cell suspension in PEB buffer at 4 °C for 15 min. .. Positive selection was performed using two sequential LS/MS separation columns (Miltenyi Biotec, Germany).

    Single Cell:

    Article Title: Targeting OxLDL-mediated CD36 + CAF reprogramming potentiates PD-1 immunotherapy in osteosarcoma.
    Article Snippet: .. For human fibroblast isolation, 600 the Human Anti-Fibroblast MicroBeads (Miltenyi Biotec, #130-050-601) were incubated with the 601 AR TIC LE IN PR ES S single-cell suspension in PEB buffer at 4°C for 15 minutes. .. Positive selection was performed using 602 two sequential LS/MS separation columns (Miltenyi Biotec, Germany).

    Article Title: Targeting OxLDL-mediated CD36 + CAF reprogramming potentiates PD-1 immunotherapy in osteosarcoma
    Article Snippet: .. For human fibroblast isolation, the Human Anti-Fibroblast MicroBeads (Miltenyi Biotec, #130-050−601) were incubated with the single-cell suspension in PEB buffer at 4 °C for 15 min. .. Positive selection was performed using two sequential LS/MS separation columns (Miltenyi Biotec, Germany).

    Selection:

    Article Title: Identification and characterization of tumor and stromal derived liquid biopsy analytes in pancreatic ductal adenocarcinoma
    Article Snippet: Levels of plasma HA were determined using the Quantikine® ELISA Hyaluronan Immunoassay (R&D Systems Inc., Minneapolis, USA) according to the manufacturer’s instructions. .. The spiked samples were further processed using two different enrichment methods: First, positive selection of CAFs from the PBMCs was performed with human Anti-Fibroblast MicroBeads (D7-FIB-conjugated, Miltenyi Biotec) using MS Columns (MACS® cell separation, Miltenyi Biotec) following the manufacturer’s instruction. .. As a comparative, marker-independent technique for processing the spiked EDTA blood, we employed the microfluidic ParsortixTM cell separation system (Angle plc, UK).

    Article Title: Identification and characterization of tumor and stromal derived liquid biopsy analytes in pancreatic ductal adenocarcinoma.
    Article Snippet: Levels of plasma HA were determined using the Quantikine® ELISA Hyaluronan Immunoassay (R&D Systems Inc., Minneapolis, USA) according to the manufacturer’s instructions. .. Spiked blood and patient sample processing for cCAF and CTC analysis The spiked samples were further processed using two different enrichment methods: First, positive selection of CAFs from the PBMCs was performed with human Anti-Fibroblast MicroBeads (D7-FIB-conjugated, Miltenyi Biotec) using MS Columns (MACS® cell separation, Miltenyi Biotec) following the manufacturer’s instruction. .. As a comparative, marker-independent technique for processing the spiked EDTA blood, we employed the microfluidic ParsortixTM cell separation system (Angle plc, UK).

    Magnetic Cell Separation:

    Article Title: Identification and characterization of tumor and stromal derived liquid biopsy analytes in pancreatic ductal adenocarcinoma
    Article Snippet: Levels of plasma HA were determined using the Quantikine® ELISA Hyaluronan Immunoassay (R&D Systems Inc., Minneapolis, USA) according to the manufacturer’s instructions. .. The spiked samples were further processed using two different enrichment methods: First, positive selection of CAFs from the PBMCs was performed with human Anti-Fibroblast MicroBeads (D7-FIB-conjugated, Miltenyi Biotec) using MS Columns (MACS® cell separation, Miltenyi Biotec) following the manufacturer’s instruction. .. As a comparative, marker-independent technique for processing the spiked EDTA blood, we employed the microfluidic ParsortixTM cell separation system (Angle plc, UK).

    Article Title: Identification and characterization of tumor and stromal derived liquid biopsy analytes in pancreatic ductal adenocarcinoma.
    Article Snippet: Levels of plasma HA were determined using the Quantikine® ELISA Hyaluronan Immunoassay (R&D Systems Inc., Minneapolis, USA) according to the manufacturer’s instructions. .. Spiked blood and patient sample processing for cCAF and CTC analysis The spiked samples were further processed using two different enrichment methods: First, positive selection of CAFs from the PBMCs was performed with human Anti-Fibroblast MicroBeads (D7-FIB-conjugated, Miltenyi Biotec) using MS Columns (MACS® cell separation, Miltenyi Biotec) following the manufacturer’s instruction. .. As a comparative, marker-independent technique for processing the spiked EDTA blood, we employed the microfluidic ParsortixTM cell separation system (Angle plc, UK).



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